Free gp70 from FeLV: enrichment from cell culture fluid by ferric oxide-agarose chromatography
Zelikman, I.; Akerblom, L.; Hjertèn, S.; Morein, B.
Biotechnology and Applied Biochemistry 11(2): 209-216
1989
ISSN/ISBN: 0885-4513 PMID: 2541738 Document Number: 337088
A new chromatographic material based on beads of macroporous crosslinked agarose containing ferric oxide particles was used for enrichment of gp70--the envelope glycoprotein of feline leukemia virus (FeLV). Free gp70 was purified from cell culture fluid in one step with a recovery of 50 to 60% and a purification of about 60 times. The described procedure is a suitable first step for the purification of gp70 from large volumes of cell culture fluid.