Bubble free cell culture aeration with porous moving membranes
Lehmann, J.; Piehl, G.W.; Schulz, R.
Developments in Biological Standardization 66: 227-240
1987
ISSN/ISBN: 0301-5149 PMID: 3582751 Document Number: 291147
For the cultivation of mammalian cells a new aeration system was developed. Its construction with hydrophobic porous Accurel hollow fiber membranes and the use in reactors with 1 and 20 l reaction volume will be presented and introduced by means of the cultivation of a genetically manipulated mouse cell line. The mass transfer capacity of this system is demonstrated as a function of gas flow rates through the membrane and of the membrane movement.