Evaluation of human sperm-zona pellucida tight binding by presence of monoclonal antibodies to sperm antigens
Mahony, M.C.; Fulgham, D.L.; Blackmore, P.F.; Alexander, N.J.
Journal of Reproductive Immunology 19(3): 269-285
1991
ISSN/ISBN: 0165-0378 PMID: 1865391 DOI: 10.1016/0165-0378(91)90040-wDocument Number: 335562
This paper reports on a study in which 26 monoclonal antibodies (MAbs) to human sperm were evaluated for their inhibition characteristics in preventing sperm-zona pellucida tight binding. Evaluation was performed using the hemizona assay (HZA) technique. Of the 26 MAbs tested, only 1 (3.9%) inhibited sperm binding to the zona pellucida. The whole sperm-specific MAb did reduce zona binding by 70%. The MAb also caused strong agglutination, but did not prevent capacitation nor calcium influx. Data were statistically evaluated using either the Student's t-test, the Chi-square analysis, or the two-way analysis of variance. Two-way analysis of variance showed a significant decrease in sperm-zona tight binding in the presence of the MAb. Motion parameters of sperm exposed to the MAb were not significantly different from untreated controls.