The use of flow cytofluorometry for determining antisperm antibodies by sperm immobilization and sperm toxicity methods
Nikolaeva, M.N.; Verbitskiĭ, M.S.; Sukhikh, G.T.
Biulleten' Eksperimental'noi Biologii i Meditsiny 114(10): 398-399
1992
ISSN/ISBN: 0365-9615 PMID: 1288702 Document Number: 400468
During antisperm antibody detection by conventional methods of immobilization and cytotoxicity investigators used flow cytometry to detect dead and/or immovable sperm cells. It was shown that after incubation of sperm cells with low antiserum dilution immovable spermatozoa are still living. Evidently, cytotoxicity test in this case brings about false-negative results. Therefore, flow cytometry will be a success for ASAB detection through immobilization test.