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Characteristics of monoclonal antibodies reactive with human sperm and seminal plasma

Gupta, S.K.; Fulgham, D.L.; Alexander, N.J.

Indian Journal of Experimental Biology 28(6): 501-507

1990

ISSN/ISBN: 0019-5189
PMID: 2401526
Document Number: 355057
The characteristics of monoclonal antibodies developed against human spermatozoa are described. Out of 10 monoclonal antibodies 9 did not react in ELISA with human RBC, WBC, platelets, Raji cells nor mouse sperm. Four monoclonal antibodies reacted with monkey sperm and all 10 reacted with human seminal plasma. Monoclonal antibodies showed differential reactivity with pre- and post-capacitated sperm. Four monoclonal antibodies were able to agglutinate sperm whereas none of these were positive in sperm-immobilization assay. Interestingly, two monoclonal antibodies (MA-46 and MA-50) were able to block the attachment of pre-capacitated sperm to zona denuded hamster oocytes. MA-46 and MA-50 recognized in immunoblot spermatozoa antigens having apparent molecular weights of 14 and 20 K Da and greater than 200 K Da respectively. The monoclonal antibodies reported in this study will be useful in further delineating the spermatozoa antigens involved in regulation of fertility.

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Shigeta, M.; Takada, Y.; Ikuma, K.; Kubota, K.; Koyama, K.; Isojima, S. 1985: Production and characterization of a monoclonal sperm immobilizing antibody to seminal plasma antigens Nihon Sanka Fujinka Gakkai Zasshi 37(10): 2031-2038
Mahony, M.C.; Fulgham, D.L.; Blackmore, P.F.; Alexander, N.J. 1991: Evaluation of human sperm-zona pellucida tight binding by presence of monoclonal antibodies to sperm antigens Journal of Reproductive Immunology 19(3): 269-285
Singer, R.; Landau, B.; Joshua, H.; Zukerman, Z.; Pick, I.; Sigienriech, E.; Chowers, I. 1976: Protein content of human seminal plasma and spermatozoa in relation to sperm counts Acta Europaea Fertilitatis 7(4): 281-284
Hu, J.-M.; Xu, C.-Y.; Li, Y.; Geng, L.; Ma, G.; Wang, L.; Chen, Z.-J. 2009: Effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm Zhonghua Nan Ke Xue 15(5): 422-424
Koistinen, H.; Seppälä, M.; Koistinen, R. 1994: Different forms of insulin-like growth factor-binding protein-3 detected in serum and seminal plasma by immunofluorometric assay with monoclonal antibodies Clinical Chemistry 40(4): 531-536
Uchigata, Y.; Prabhakar, B.S.; Salata, K.F.; Ginsberg-Fellner, F.; Notkins, A.L. 1987: Human monoclonal multiple-organ-reactive autoantibodies distinguished by mouse monoclonal anti-idiotypic antibodies: expression of idiotopes in humans with and without autoimmune diseases Journal of Immunology 138(12): 4218-4221
Dimitrova, D.; Kiurkchiev, S. 1986: Antigens of human sperm studied using monoclonal antibodies Akusherstvo i Ginekologiia 25(5): 75-80
Isojima, S.; Koyama, K.; Fujiwara, N. 1982: Purification of human seminal plasma no. 7 antigen by immunoaffinity chromatography on bound monoclonal antibody Clinical and Experimental Immunology 49(2): 449-456
Yamada, K.; Okabe, N.; Saito, H.; Suzuki, R.; Kumagai, K. 1983: Monoclonal antibodies reactive with human granulocytes and monocytes Nihon Ketsueki Gakkai Zasshi: Journal of Japan Haematological Society 46(6): 1185-1191
Wisnewski, A.V.; Olds, G.R.; Kresina, T.F. 1994: Human monoclonal antibodies heterogeneously express a human cross-reactive idiotype associated with immune function in Schistosoma japonicum infection Human Antibodies and Hybridomas 5(3-4): 178-182
Quinlivan, W.L.; Sullivan, H. 1977: Spermatozoal antibodies in human seminal plasma as a cause of failed artificial donor insemination Fertility and Sterility 28(10): 1082-1085
Gupta, G.S.; Agarwal, P. 1982: Immunological characteristics of beta-N-acetylglucosaminidase from human seminal plasma Indian Journal of Biochemistry and Biophysics 19(1): 37-40
Soon-Shiong, P.; Heintz, R.; Terasaki, P. 1989: Identification of novel blood group-reactive monoclonal antibodies cytotoxic to human acinar cells but not islets Transplantation Proceedings 21(1 Pt 3): 2622-2623
Caraux, J.; Chichehian, B.; Gestin, C.; Longhi, B.; Lee, A.C.; Powell, J.E.; Stevens, V.C.; Pourquier, A. 1985: Non-cross-reactive monoclonal antibodies to human chorionic gonadotropin generated after immunization with a synthetic peptide Journal of Immunology 134(2): 835-840
Fendly, B.M.; Winget, M.; Hudziak, R.M.; Lipari, M.T.; Napier, M.A.; Ullrich, A. 1990: Characterization of murine monoclonal antibodies reactive to either the human epidermal growth factor receptor or HER2/neu gene product Cancer Research 50(5): 1550-1558
Furukawa, K.; Hayashi, K.; Naoe, T.; Takamoto, S.; Shiku, H.; Yamada, K. 1987: Monoclonal antibodies reactive to human platelets: analysis of their specificities, detected antigen molecules, and their effects on platelet function Nihon Ketsueki Gakkai Zasshi: Journal of Japan Haematological Society 50(4): 914-925
Burchell, J.; Gendler, S.; Taylor-Papadimitriou, J.; Girling, A.; Lewis, A.; Millis, R.; Lamport, D. 1987: Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin Cancer Research 47(20): 5476-5482
Furukawa, K.; Yamaguchi, H.; Oettgen, H.F.; Old, L.J.; Lloyd, K.O. 1989: Two human monoclonal antibodies reacting with the major gangliosides of human melanomas and comparison with corresponding mouse monoclonal antibodies Cancer Research 49(1): 191-196
Friedman, S.M.; Hunter, S.B.; Irigoyen, O.H.; Kung, P.C.; Goldstein, G.; Chess, L. 1981: Functional analysis of human T cell subsets defined by monoclonal antibodies. II. Collaborative T-T interactions in the generation of TNP-altered-self-reactive cytotoxic T lymphocytes Journal of Immunology 126(5): 1702-1705
Keen, J.; Chang, S.E.; Taylor-Papadimitriou, J. 1984: Monoclonal antibodies that distinguish between human cellular and plasma fibronectin Molecular Biology and Medicine 2(1): 15-27