Monoclonal antibodies that distinguish between human cellular and plasma fibronectin
Keen, J.; Chang, S.E.; Taylor-Papadimitriou, J.
Molecular Biology and Medicine 2(1): 15-27
1984
ISSN/ISBN: 0735-1313 PMID: 6083431 Document Number: 229010
Two monoclonal antibodies have been developed against human fibronectin expressed by simian virus 40 transformed human mammary epithelial cells. Monoclonal antibody ICRF-FN-3 recognizes a determinant on human cellular but not plasma fibronectin. The antigenic site recognized by FN-3 is lost after limited proteolysis of cellular fibronectin with alpha-chymotrypsin but not trypsin. Monoclonal antibody, ICRF-FN-4, against a determinant on both human plasma and cellular fibronectin reacts with an antigenic site on fibronectin which is not destroyed by trypsin or alpha-chymotrypsin. Although the epitopes recognized by FN-3 and FN-4 are expressed similarly by cells from the human breast they are expressed differently on rat mammary (RAMA) cell lines.