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Purification and identification of ADP-ribosylated proteins from bull testis intact nuclei

Faraone-Mennella, M.R.; Raucci, A.; Leone, E.; Farina, B.

Biochemistry International 19(6): 1265-1275

1989

ISSN/ISBN: 0158-5231
PMID: 2517579
Document Number: 335233
Isolated, intact bull testis nuclei were incubated with [14C] NAD. A large amount of radioactivity was associated to loosely bound chromosomal proteins extracted with 0.35M NaCl and fractionated with trichloroacetic acid. The labelled nuclear proteins included essentially a number of components belonging to the low mobility group. Mg2(+)-catalyzed alkali digestion of radioactive proteins and further analysis demonstrated that the final products were 5'-AMP and phospho-ribosyl-AMP, which arise from the hydrolysis of poly(ADP-ribose).

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