A new high-yield continuous cell culture system and its clinical application in adoptive immunotherapy with LAK cells
Noto, T.; Tokuda, Y.; Tajima, T.; Mitomi, T.; Nakamura, Y.; Watanabe, K.; Yamamura, M.; Ichinohe, K.
Gan to Kagaku Ryoho. Cancer and ChemoTherapy 16(4 Pt 2-3): 1893-1898
1989
ISSN/ISBN: 0385-0684 PMID: 2658834 Document Number: 329613
We developed a high-yield culture system, consisting of a culture bag on a rotator. The culture bag has two compartments, an inner compartment separated from an outer compartment by a semipermeable membrane. Cells in an appropriate culture medium are placed in the inner compartment, and medium without serum is placed in the outer compartment. The bag is rotated at an angle of 45 degrees between 0.5 and 5 rpm in a 37 degrees C incubator. The medium is changed in the outer compartment at intervals when needed. The outer compartment acts as the feeder as well as allows catabolites to diffuse through the culture. In addition, since the system is a closed-system, sterile conditions can easily be maintained. Using this system, we demonstrated that up to 20 X 10(6) cells/ml of PBMC could be cultured in IL-2 with sufficient recovery rate and cytotoxicity.