Degradation of messenger RNA, ribosomal RNA and 2-5A-dependent inhibition of protein biosynthesis
Toots, U.E.; Kel've, M.B.; Saarma, M.I.
Molekuliarnaia Biologiia 22(6): 1473-1481
1988
ISSN/ISBN: 0026-8984 PMID: 2472548 Document Number: 324593
In rabbit reticulocyte lysates the addition of exogenous 2-5A leads after 10-20 minutes to the inhibition of protein synthesis. This inhibition can be blocked by rat antiserum to 2-5A. In intact ribosomes the ribosomal RNA is cleaved after 2-5A addition, but this cleavage is not in correlation with the protein synthesis shutoff. Ribosomal 5S RNA and 5,8S RNA are not cleaved even after several hours of incubation with 2-5A. The degradation of polysome associated mRNA correlates with the protein synthesis inhibition as revealed by Northern blot hybridization of globin mRNA with 32P-labelled p beta G plasmid. The addition of 2-5A antiserum to the rabbit reticulocyte lysate also inhibits the degradation of polysome bound globin mRNA.