Kinetic properties of poly (2'-O-methylcytidylic acid) -directed reverse transcriptase reaction
Aoyama, H.
Brazilian Journal of Medical and Biological Research 21(3): 445-448
1988
ISSN/ISBN: 0100-879X PMID: 2465802 Document Number: 322492
Poly (2'-O-methylcytidylic acid) is recognized as a template in reactions catalyzed by RNA-dependent DNA polymerases in the presence of Mn2+ as divalent cation. We report that kinetic data obtained for dGTP and template under optimal experimental conditions in the reaction catalyzed by reverse transcriptase showed some similarities between the poly (2'-O-methylcytidylic acid)/Mn2+ and polyribocytidylic acid/Mg2+ systems. The reaction was inhibited by the action of N-ethylmaleimide and novobiocin, and to a lesser extent by ethidium bromide and tetramethyl ethidium bromide.
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