Effective inactivatian test of inactivated hepatitis a vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction
Zhou, J.; Ji, G.; Wen, J.-n.; Li, J.; Sheng, W.; Guo, Z.-q.; Liao, G.-y.; Jiang, S.-d.; Sun, M.-b.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 22(6): 488-491
2008
ISSN/ISBN: 1003-9279 PMID: 19544655 Document Number: 616995
To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine. effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay. all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR. ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.