Isolation, characterization, and molecular cloning of cryptic plasmids isolated from Edwardsiella ictaluri
Newton, J.C.; Bird, R.C.; Blevins, W.T.; Wilt, G.R.; Wolfe, L.G.
American Journal of Veterinary Research 49(11): 1856-1860
1988
ISSN/ISBN: 0002-9645 PMID: 3247906 Document Number: 318175
Fifty-five isolates of E. ictaluri were examined for plasmid DNA by a rapid alkaline extraction procedure. All 49 isolates from channel catfish and a single isolate from Bengal danio carried 2 plasmids with molecular masses of approximately 3.2 and 3.7 megadaltons (Mdal). Five E. ictaluri isolates from other fish contained 1 to 3 plasmids, which had molecular masses ranging from 2.5 to 45 Mdal. The 2 plasmids (3.2 and 3.7 Mdal) from the type strain of E. ictaluri (ACTCC 33202) were ligated into pUC19 cloning vectors, and restriction endonuclease maps of each insert were prepared.