Molecular weights of the hetero-organic NHCP antigens of kidney origin associated with rat hepatomas
Kushner, V.P.; Grandilevskaia, A.B.; Ivanov, V.A.
Tsitologiia 30(10): 1226-1231
1988
ISSN/ISBN: 0041-3771 PMID: 3266688 Document Number: 314634
The narrow NHCP protein fractions, possessing a proper phosphoprotein kinase activity, were isolated from kidney of intact rats, hepatoma tissue and liver cells of rats treated with hepatocarcinogen in the process of phosphocellulose gradient chromatography by elution of 0.4-0.5 M NaCl. The gel filtration on Sephadex G-75 and SDS-PAAG electrophoretic data demonstrate that all the NHCP protein fractions mentioned above include a general molecular component with the mass of 23 kD, and display identical antigenic properties. Thus, in accordance with the data obtained, the role of the hetero-organic NHCP protein antigen of kidney origin associated with hepatoma may be played by the general molecular component of NHCP protein fractions possessing properties of a specific chromosomal phosphoprotein kinase.