Evaluation of molecular weights of hyaluronate preparations by multi-angle laser light scattering
Yomota, C.
Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo Hokoku 121: 30-33
2003
ISSN/ISBN: 1343-4292 PMID: 14740402 Document Number: 558519
Hyaluronate (HA), a glycosaminoglycan polysaccharide, has been used for osteoarthritis, periartritis of the shoulder and rheumatoid arthritis by intraarticular administration, and in ophthalmic surgery such as anterior segment surgery, and eye lotion. In this study, the molecular weight (Mw) of HA preparations were estimated by size-exclusion chromatography (SEC) system consisted of a refractometer (RI) and a multi-angle laser light scattering (MALS). From the results, it has been clarified that a successful characterization of HA samples with Mw up to 2 - 3 x 10(6) g/mol was possible by multidetector system.