Cytophotometric analysis of the apoprotein B content of sections of the human aorta
Babaev, V.R.; Osis, I.L.; Vikhert, A.M.
Biulleten' Eksperimental'noi Biologii i Meditsiny 105(6): 743-746
1988
ISSN/ISBN: 0365-9615 PMID: 2455564 Document Number: 312225
A cytophotometric technique to quantitate the content of apolipoprotein B (apo B) in cryostate sections of human aorta is described. The method is based on the comparison of different regions of the sections stained by indirect immunoperoxidase technique, using antibody to apo B. It has been found that the optical density of stained intimal layer was 8-22 times higher than that of the stained aortic media, which in its turn, differed from the control values. The intensity of specific staining of fibrous plaques was 58-87% of the staining intimal tissue of the apparently unaffected intima. About 25-42% of apo B content was extracted from the sections of the normal part of intima and fibrous plaque tissue during incubation of nonfixed sections in a physiologic saline.