Effect of Prompt Inoculator on results of Sceptor system minimal inhibitory concentration determinations for Listeria monocytogenes
Dillon, P.A.; Overman, T.L.
Archives of Pathology and Laboratory Medicine 112(2): 163-165
1988
ISSN/ISBN: 0003-9985 PMID: 3122704 Document Number: 308835
The minimal inhibitory concentrations (MICs) of eight antimicrobial agents were determined for each of 36 isolates of Listeria monocytogenes with the Sceptor system. Two different inoculation procedures, the standard Sceptor log-phase broth (LB) culture and the Prompt Inoculator (PI) (Bauer-Kirby type), were used. The PI MIC/LB MIC ratio (PI/LB ratio) was determined for each antimicrobial agent for each of the isolates. Of the 217 on-scale PI/LB ratio results, all were within the expected range of 0.5 to 2.0. The PI was found to be a convenient, rapid, and suitable method of preparing an inoculum of L. monocytogenes for use with the Sceptor system and should function equally well when testing L. monocytogenes isolates with other commerically available MIC systems.