Relaxation phenomena in systems of protein-containing reverse micelles of surfactants in organic solvents
Kabanov, A.V.; Nametkin, S.N.; Matveeva, E.G.; Kliachko, N.L.; Martinek, K.
Molekuliarnaia Biologiia 22(2): 473-484
1988
ISSN/ISBN: 0026-8984 PMID: 2455866 Document Number: 308622
The research was aimed to establish the equilibrium processes in protein-containing systems of AOT reverse micelles in octane. As chromophore label for tracing the kinetics of the process, the acid-base indicator, p-nitrophenol, was used. The establishing of the equilibrium in the reverse micelle system notably decelerated in the presence of a solubilized protein (native and stearoylated alpha-chymotrypsin). During the establishing of the equilibrium, the solubilized enzyme can be irreversibly inactivated. The level of the residual activity of the enzyme in the equilibrium system depended on the procedure of micellar system preparation. The methods have been offered to set up the equilibrium in the reverse micelle system without inactivation of the solubilized enzyme.