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Isolation and characterization of a specific antibody population against human fibrinogen

Lau, H.K.; Cheung, M.; Siu, A.

Biochemistry International 15(3): 561-569

1987

ISSN/ISBN: 0158-5231
PMID: 2447894
Document Number: 292690
A specific antibody population against human fibrinogen was isolated from a rabbit antiserum by affinity chromatography on fibrinogen-bound Sepharose gel. Using a sensitive competitive radioimmunoassay, the antibody population was found to recognize epitopes on native fibrinogen but crossreacted minimally with fibrinogen fragment D and an early plasmin-degraded fibrinogen A.alpha.-chain product, but not at all with fragment E or fibrinopeptides A and B. Fibrin monomers shared part of these epitopes. The antibody population crossreacted to a small extent with bovine, horse and baboon fibrinogens and not at all with fibrinogens from sheep, rat, pig, goat, guinea pig, dog and rabbit.

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