Characterization of the humoral immune response in Sudanese leishmaniasis: specific antibody detected by class- and subclass-specific reagents
El Amin, E.M.; Wright, E.P.; Vlug, A.
Clinical and Experimental Immunology 64(1): 14-19
1986
ISSN/ISBN: 0009-9104 PMID: 3731524 Document Number: 284825
Sera from Sudanese patients with either visceral or mucosal manifestations of infection with Leishmania were investigated to determine the class of antibodies against the causative parasite in an enzyme-linked immunosorbent assay (ELISA) with intact promastigotes as antigen. All patient sera had significantly more IgG and more antiparasite IgG than did control Sudanese or control Dutch sera; little or no IgM or IgA antiparasite antibody activity was detected. When sera specific for the subclasses of IgG were used to detect the anti-promastigote antibodies, these were found in IgG1- and IgG3-specific ELISA but not in those for IgG2 or IgG4.