A monoclonal antibody to myelogenous leukemia: isolation and characterization
Malcolm, A.J.; Shipman, R.C.; Logan, P.M.; Levy, J.G.
Experimental Hematology 12(7): 539-547
1984
ISSN/ISBN: 0301-472X PMID: 6589167 Document Number: 232766
A purified antigen from human acute myelogenous leukemia (AML) cells was used to produce a myelogenous leukemia-associated monoclonal antibody. In limited FACS[fluorescence-activated cell sorter]-IV analyses the monoclonal antibody to leukemia (CAMAL-1) as well as a conventional rabbit antiserum were used to positively identify AML or chronic granulocytic leukemia patient cell samples. Neither CAMAL-1 nor the rabbit antiserum bound appreciably to acute lymphocytic leukemia cells, normal bone marrow, or normal peripheral blood leukocytes. CAMAL-1 was shown to be specific for AML cell extracts in the ELISA [enzyme-linked immunosorbent assay] and was successfully used as an immunoadsorbent for the purification of the AML antigen from cell extracts. No significant levels of equivalent antigen were found when cell extracts from normal cells, lymphocytic leukemia cells, and lymphoma cells were similarly absorbed. CAMAL-1 shows considerable specificity for an antigen associated with cells from patients with myelogenous leukemia.