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Production and characterization of gold labeled monoclonal antibody against MC-LR

Zhao, X.-l.; Sun, W.-r.; Sun, X.-l.; Jiang, T.

Wei Sheng Yan Jiu 35(4): 442-445

2006

ISSN/ISBN: 1000-8020
PMID: 16986520
Document Number: 598695
Production of the gold labeled monoclonal antibody against MC-LR. Study the simple mechanism of the conjugation by the spectral analysis. Production and purification monoclonal antibodies against MC-LR by hybridoma technology. Production the 20nm colloidal gold and label the monoclonal antibody. Study the gold--McAb conjugate by the spectroanalysis. Monoclonal antibody produced by the hybridoma cells were tested for subtypes and designated as IgG2a for 8C11. The titers of antibody in ascites was 108.The IC50 for MC-LR was around 3ng/ml. The IgG molecule weight was 1.5 x 10(5). The structure of the antibody was altered to more tighter and stabler. Product the gold labeled monoclonalantibody against MC-LR conjugate successfully and keep the antige-binding determinant.

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Xue, Y.; Sun, C.; Tan, J. 1995: Production and characterization of specific monoclonal antibody against Porphyromonas endodontalis Zhonghua Kou Qiang Yi Xue Za Zhi 30(6): 343-345; 384
Andrews, G.A.; Chavey, P.S.; Smith, J.E. 1992: Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1 Journal of the American Veterinary Medical Association 201(10): 1549-1552
Heinrichs, M.; Jacobasch, G.; Scheiner-Bobis, K.; Bertram, S.; Eigenbrodt, E.; Reinacher, M. 1987: Human erythrocyte pyruvate kinase (L'/R-PK): production and characterization of a monoclonal antibody Biomedica Biochimica Acta 46(2-3): S223-S228
Shigeta, M.; Takada, Y.; Ikuma, K.; Kubota, K.; Koyama, K.; Isojima, S. 1985: Production and characterization of a monoclonal sperm immobilizing antibody to seminal plasma antigens Nihon Sanka Fujinka Gakkai Zasshi 37(10): 2031-2038
Kumar, A.; Kurup, V.P.; Greenberger, P.A.; Fink, J.N. 1993: Production and characterization of a monoclonal antibody to a major concanavalin A--nonbinding antigen of Aspergillus fumigatus Journal of Laboratory and Clinical Medicine 121(3): 431-436
Intrarapuk, A.; Awakairt, S.; Thammapalerd, N.; Mahannop, P.; Pattanawong, U.; Suppasiri, T. 2001: Comparison between R-phycocyanin-labeled and R-phycoerythrin-labeled monoclonal antibody (Mab) probes for the detection of Entamoeba histolytica trophozoites Southeast Asian Journal of Tropical Medicine and Public Health 32(Suppl 2): 165-171
Fukaya, T. 1991: Mouse monoclonal antibody (FKH1) detecting human melanoma-associated antigens: production, partial characterization and immunohistochemical analysis Hokkaido Journal of Medical Science 66(3): 300-310
Hegyi, E.; Nakeff, A. 1989: Ultrastructural localization of platelet factor 4 in rat megakaryocytes and platelets by gold-labeled antibody detection Experimental Hematology 17(3): 223-228
Juhl, H.; Sievers, M.; Baltzer, K.; Helmig, F.; Wolf, H.; Brenner, W.; Kalthoff, H. 1995: A monoclonal antibody-cobra venom factor conjugate increases the tumor-specific uptake of a 99mTc-labeled anti-carcinoembryonic antigen antibody by a two-step approach Cancer Research 55(23 Suppl): 5749s-5755s
Woodhouse, C.S.; Morgan, A.C. 1989: Murine monoclonal IgG3 antibodies to human colorectal tumor-associated antigens: production and characterization of antibodies active in both antibody-dependent cellular cytotoxicity and complement-mediated cytolysis Cancer Research 49(10): 2766-2772
Kusakabe, K.; Yamazaki, T. 1985: Radionuclide imaging with labeled monoclonal antibody Nihon Rinsho. Japanese Journal of Clinical Medicine 43(4): 860-869
Dudczak, R.; Kletter, K.; Bailer, H.; Kokoschka, E.; Pohl-Markl, H. 1987: Immunoscintigraphy with a technetium 99m-labeled monoclonal antibody in patients with melanoma Wiener Klinische Wochenschrift 99(7): 232-239
Jones, P.L.; Brown, B.A.; Sands, H. 1990: Uptake and metabolism of 111In-labeled monoclonal antibody B6.2 by the rat liver Cancer Research 50(3 Suppl): 852s-856s
Major, P.P.; Dion, A.S.; Williams, C.J.; Mattes, M.J.; Wang, T.; Rosenthall, L. 1990: Breast tumor radioimmunodetection with a 111In-labeled monoclonal antibody (MA5) against a mucin-like antigen Cancer Research 50(3 Suppl): 927s-931s
Macey, D.J.; Denardo, S.J.; Denardo, G.L.; Goodnight, J.K.; Unger, M.W. 1988: Uptake of indium-111-labeled monoclonal antibody ZME-018 as a function of tumor size in a patient with melanoma American Journal of Physiologic Imaging 3(1): 1-6
Matsumura, H.; Otsuji, E.; Kobayashi, S.; Okamoto, K.; Toma, A.; Yamagishi, H. 2001: Potential usefulness of 99mTc-labeled monoclonal antibody A7 for immunoscintigraphy of human pancreatic carcinoma Anticancer Research 21(5): 3433-3437
Schlom, J.; Siler, K.; Milenic, D.E.; Eggensperger, D.; Colcher, D.; Miller, L.S.; Houchens, D.; Cheng, R.; Kaplan, D.; Goeckeler, W. 1991: Monoclonal antibody-based therapy of a human tumor xenograft with a 177lutetium-labeled immunoconjugate Cancer Research 51(11): 2889-2896
Murray, J.L.; Lamki, L.M.; Shanken, L.J.; Blake, M.E.; Plager, C.E.; Benjamin, R.S.; Schweighardt, S.; Unger, M.W.; Rosenblum, M.G. 1988: Immunospecific saturable clearance mechanisms for indium-111-labeled anti-melanoma monoclonal antibody 96.5 in humans Cancer Research 48(15): 4417-4422
Woo, D.V.; Li, D.; Mattis, J.A.; Steplewski, Z. 1989: Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells Cancer Research 49(11): 2952-2958
Blend, M.J.; Hyun, H.; Kozloff, M.; Levi, H.; Mills, G.Q.; Gasparini, M.; Buraggi, G.; Hughes, L.; Pinsky, C.M.; Goldenberg, D.M. 1995: Improved staging of B-cell non-Hodgkin's lymphoma patients with 99mTc-labeled LL2 monoclonal antibody fragment Cancer Research 55(23 Suppl): 5764s-5770s