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Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1

Andrews, G.A.; Chavey, P.S.; Smith, J.E.

Journal of the American Veterinary Medical Association 201(10): 1549-1552

1992

ISSN/ISBN: 0003-1488
PMID: 1289333
Document Number: 191
A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (DEA) 1.1, has been produced. The antibody correctly identified canine BBC possessing DEA 1.1 in a panel of RBC typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-DEA 1.1 antiserum with 163 RBC samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for DEA 1.1 in clinical practice.

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Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1

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