Characterization of human anti-hrB-like monoclonal antibody
Blancher, A.P.; Reid, M.E.; Alié-Daram, S.J.; Dugoujon, J.M.; Roubinet, F.L.
Immunohematology 12(3): 119-122
1996
ISSN/ISBN: 0894-203X PMID: 15387737 Document Number: 457613
To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R2R2, RZRZ, Dc-, D--, Rhnull, and e+ hrB-. The supernatant is useful as a reagent to screen for hrB- blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.