Detection of tissue-specific gene expression using an in situ hybridization method of histoblotting
Kashkin, K.N.; Troianovskiĭ, S.M.; Gudkov, A.V.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 9: 16-18
1987
ISSN/ISBN: 0208-0613 PMID: 2448614 Document Number: 292256
A version of in situ hybridization on the histological sections that is used for screening specific mRNA in tissues in proposed. Sections of the frozen tissue samples are prepared on the cryostatic microtome, placed on nitrocellulose filters and hybridized with labelled DNA-probes under the conditions of RNA blot hybridization. The proposed method ("histoblotting") was used to study the distribution of actin and alpha-phetoprotein mRNA genes in tissues of 15 and 21-day rat embryos. The possibility of studying mRNA (by hibridizations) and protein (by immunoenzyme staining) and making histological analysis simultaneously by histoblotting is demonstrated.