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Comparative detection of herpesviruses in tissue specimens by in situ hybridization and immunofluorescence

Sauerbrei, A.; Wutzler, P.; Färber, I.; Brichácek, B.; Swoboda, R.; Macheleidt, S.

Acta Virologica 30(3): 213-219

1986

ISSN/ISBN: 0001-723X
PMID: 2874725
Document Number: 278920
The conditions of in situ hybridization for demonstration of herpesvirus genomes in animal and human tissues were tested using the ORWO(R) K6 emulsion. It was possible to localize herpes simplex virus (HSV) genomes in infected mice organs (brain and liver) as well as Epstein-Barr virus (EBV) genomes in tonsils of patients with infectious mononucleosis and in tumour specimens of patients with nasopharyngeal carcinomas. Immunofluorescence (IF) revealed mostly corresponding results. The in situ hybridization is most favourable due to it's higher specificity, but it is more time consuming and expensive. IF seems advantageous for screening of a great number of tissues.

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