The role of tryptophan residues of NADPH-adrenodoxin reductase in the formation of complex with adrenodoxin
Sarkisova, E.G.; Mardanian, S.S.
Biokhimiia 52(8): 1258-1262
1987
ISSN/ISBN: 0320-9725 PMID: 3663762 Document Number: 286792
Chemical modification of tryptophan residues by N-bromosuccinimide was used to determine the role of these residues in the NADPH-adrenodoxin-catalyzed reduction of adrenodoxin, dichlorophenolindophenol and ferricyanide. It was shown that the rate of reduction of all electron acceptors diminishes with modification of tryptophan residues. The most significant decrease of the enzyme activity is observed in case of adrenodoxin-catalyzed reactions. It was suggested that tryptophan residues are responsible for the adrenodoxin reductase interaction with adrenodoxin.