Nitroreductase reactions of the NADPH: adrenodoxin reductase and the adrenodoxin complex
Marcinkeviciene, J.; Cenas, N.; Kulys, J.; Usanov, S.A.; Sukhova, N.M.; Selezneva, I.S.; Gryazev, V.F.
Biomedica Biochimica Acta 49(4): 167-172
1990
ISSN/ISBN: 0232-766X PMID: 2403337 Document Number: 365161
NADPH: adrenodoxin reductase (E.C. 1.18.1.2) and its complex with adrenodoxin catalyze the aerobic oxidation of NADPH by a number of substituted 2-nitrofurans, 5-nitroimidazoles and p-derivatives of nitrobenzene. The nitrocompounds are reduced via an initial single-electron transfer. Under anaerobic conditions nitrofurans are reduced to the corresponding amines. The rate constants of adrenodoxin oxidation by nitrocompounds vary from 4 .cntdot. 105 to 3 .cntdot. 102 M-1 s-1. A linear correlation between the rate constant logarithm and the single-electron reduction potential at pH 7.0 (E17) of nitrocompounds was observed. The relation between the reactivity and the polarographic half-wave potential (E1/27) is distorted. The reactivity of adrenodoxin reductase is two orders of magnitude lower than that of adrenodoxin.