Purification and some properties of NADPH-cytochrome P-450 reductase from crab-eating monkey liver microsomes
Ohmori, S.; Kitada, M.; Hamada, A.; Igarashi, T.; Ueno, K.; Kanakubo, Y.; Kitagawa, H.
Biochemistry International 17(2): 249-256
1988
ISSN/ISBN: 0158-5231 PMID: 3142477 Document Number: 308587
NADPH-cytochrome P-450 reductase was purified to 30.8 units/mg from monkey liver microsomes. The purified reductase showed one major protein band (78,000) and two minor ones (58,000 and 20,000) on analysis by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Monkey, rat, and guinea pig reductases were not immunochemically identical to each other judged from Ouchterlony double diffusion analysis and immunotitration with regard to NADPH-cytochrome c reductase activity.