Host proteins in purified concentrations of the influenza virus
Popov, V.F.; Lonskaia, N.I.; Bizhanov, G.I.; Vorob'ev, A.A.; Novikov, E.V.
Voprosy Virusologii 31(3): 297-300
1986
ISSN/ISBN: 0507-4088 PMID: 3765555 Document Number: 275265
A comparative study of three methods for purification and concentration of influenza virus (adsorption on and elution from formalin-treated erythrocytes, sorption method, and purification on nuclear filters) demonstrated a significant decreased in ovalbumin content. By this criterion, all the three methods of preliminary purification yield the final preparation with a similar ovalbumin content. A more detailed study of the protein composition of influenza virus concentrates showed purification by elution from formalin-treated erythrocytes to remove greated amounts of protein admixtures. Electrophoregrams of virus concentrates produced by the adsorption method using macropore glass 8000 revealed a protein which passed into the virus suspension in sufficiently large amounts. This protein was identified as conalbumin.