Host range of murine sarcoma virus, its host modification and phenotypic mixing with endogenous virus
Sláviková, K.; Altaner, C.
Neoplasma 28(2): 159-169
1981
ISSN/ISBN: 0028-2685 PMID: 6265817 Document Number: 170605
Mouse M(MSV)AG-50 cells produce competent ecotropic sarcoma virus which is able to transform several lines of rodent embryo cells and replicate in them. The expanded host range showed that the virus acquired some host information which facilitates the transformation of heterologous cells. The transformed cells were XC positive and with transformed phenotype in vitro. The genome of mouse sarcoma virus from nonproducer rat liver cells could be rescued by xenotropic endogenous virus. The obtained virus showed the augmented host range for various embryo cells and for some mammalian cell lines as well. The virus with xenotropic coat efficiently transforms the cells, yielding cells with transformed phenotype. The majority of the viruses were phenotypically-mixed virions, with probably a minority of recombinant viruses as suggested by XC test. The modification of the virus during the passage through heterologous cells is discussed.