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Propagation and morphologic phenotypes of human umbilical cord artery endothelial cells

van Hinsbergh, V.W.; Mommaas-Kienhuis, A.M.; Weinstein, R.; Maciag, T.

European Journal of Cell Biology 42(1): 101-110

1986

ISSN/ISBN: 0171-9335
PMID: 3539606
Document Number: 272756
Human umbilical cord artery endothelial cells can be propagated on fibronectin-coated dishes for approximately 50 cumulative population doublings in the presence of crude preparations of endothelial cell growth factor (ECGF) and serum. The cells were characterized by immunofluorescent staining and their ultrastructure. Different morphologic phenotypes could be demonstrated: (1) closely attached cell monolayers, (2) atypical cells, (3) giant cells, (4) tube-like structures. The formation of tube-like structures can be induced by proteolytic modification of fibronectin. Our data demonstrate that umbilical arteries may provide an excellent source for the routine serial cultivation of human arterial endothelial cells.

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