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Method of treating leukocytes for electron microscopic research

Pal'tsyn, A.A.; Chervonskaia, N.V.; Badikova, A.K.; Uchaneishvili, E.K.

Biulleten' Eksperimental'noi Biologii i Meditsiny 101(3): 372-374

1986

ISSN/ISBN: 0365-9615
PMID: 3513864
Document Number: 269352
A technique of pretreatment of leukocytes and other cellular suspensions for the electron microscopic studies in suggested. Cells fixed in a suspension are embedded in a warm gelatine solution. Upon cooling, a gelatinous block that can be cut into small pieces is formed. These pieces are fixed in glutaraldehyde solution and used thereafter as pieces of dense tissues. The technique provides a better preservation of leukocyte structure, considerably facilitating ultratomy.

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