Lipid peroxidation and renal injury in renal ischemic reperfusion: effect ofangiotensin inhibition
Seujang, Y.; Kittikowit, W.; Eiam-Ong, S.; Eiam-Ong, S.
Journal of the Medical Association of Thailand 89(10): 1686-1693
2006
ISSN/ISBN: 0125-2208 PMID: 17128845 Document Number: 2646
Objectives: To investigate the role of angiotensin inhibition on lipid peroxidation (LPO) and renal pathology in ischemic reperfusion (IR).Material and Method. Male Wistar rats weresubjectedto 15-,30-, 45- or 60- minutes of renal ischemia (I) by left renal artery occlusion. In the 30-minute 1 group, reperfusion (R) for 1 day (I-30 R-l) was performed in additional animals that had been treated with water angiotensin converting enzyme inhibitor (ACEI; enalapril 5 mg/kg/d), or angiotensin receptor type 1 blocker (ARB; losartan 10 mglkgld) one day before I and were continued for 1 day after R. Renal tissue malondialdehyde (MDA), an indicator of LPO, was examined during I and IR periods. Renal pathology was also determined.Results: During ischemia, renal tissue MDA levels were increased throughout the 60-minute ischemic period and was maximum at 30 minutes of ischemia (p < 0.01). Histological changes in 30-minutes I group showed slight tubular cell congestion and mild interstitial edema. One day after reperfusion, MDA levels were still elevated (p < 0. 01) when compared with sham. Progression of renal pathology was observed after I day of reperfusion. Both ACEI and ARB could attenuate the heightened MDA levels (p < 0. 01). IR-induced renal injury was markedly diminished by administration of ACEI as well as by ARB.Conclusion: These results indicate that inhibition of angiotensin could reduce lipidperoxidation and ameliorate renal injury during IR condition.
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