Effect of anti-P-selectin monoclonal antibody on renal ischemia/reperfusion injury in rats
Zhou, T.; Li, X.; Wu, P.; Zhang, D.; Zhang, M.; Chen, N.; Dong, D.
Chinese Medical Journal 113(9): 790-793
2000
ISSN/ISBN: 0366-6999 PMID: 11776071 Document Number: 516625
To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats. Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used. Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group. Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.