Inhibition of human stomach cancer metastasis in vivo by anti-P-selectin monoclonal antibody
Chen, J.; Zhang, Y.; Chu, Y.
Zhonghua Yi Xue Za Zhi 78(6): 437-439
1998
ISSN/ISBN: 0376-2491 PMID: 10923507 Document Number: 492041
To study the role of cell adhesion molecule P-selectin monoclonal antibody (Mab) in tumor metastasis of an orthotopic implantation metastatic model. SCID mice were implanted orthotopically using SGC-7901 human stomach carcinoma intact tissue. Three days later, animals were given i.v. injections of PBS or P-selectin Mab (100 micrograms/injection) twice weekly for 3 weeks. 42 days after operation, all animals were sacrificed. Tissues from stomach and other organs were obtained for histopathological evaluation. Ten of the animals (n = 11) treated with PBS developed metastatic tumors in the regional lymph nodes, liver, and lung. In contrast, 2 of the animals (n = 9) treated with P-selectin Mab developed metastatic tumors in the organs examined. The level of P selectin mRNA expression determined by RT-PCR in gastric carcinoma tissue of SCID mice with tumor metastas is was higher than that without metastasis. The expression of P-selectin adhesion moleculeis associated with tumor metastasis, and the metastasis can be arrested by the Mab.