Use of permeabilized cells in the study of inhibitory products of herpes virus replication
Baginski, I.; Colson, G.; Lavelle, F.; Zerial, A.
Pathologie-Biologie 33(5 Part 2): 618-622
1985
ISSN/ISBN: 0369-8114 PMID: 3003658 Document Number: 249848
Cells made permeable by exposure to lysolecithin following infection by HSV-1 synthesize DNA (in greater amounts than non-infected cells) in the presence of the four deoxyribonucleoside-triphosphates (dNTPs) : dATP, dCTP, dGTP, and dTTP. DNA synthesis also occurs if dTTP is replaced by dT or dTMP, indicating activity of enzymes such as thymidine kinase, thymidylate kinase, deoxyribonucleoside-diphosphate kinase and ADN polymerase. Examination of DNA synthesis in permeabilized cells enables detection of antiviral activity of agents incapable of penetrating into intact cells and therefore ineffective in cell cultures. No detectable protein-tyrosine kinase activity was found in HSV-1 infected cells.