The effects of S9 mix from rat oesophagus, salivary gland, and liver on the mutagenicity of the rat oesophageal carcinogen N-nitroso-N-methylaniline in the Salmonella typhimurium assay
Anderson, D.; Blowers, S.D.; Craddock, V.M.
Mutation Research 142(1-2): 13-18
1985
ISSN/ISBN: 0027-5107 PMID: 3883137 Document Number: 246395
The present study examines the feasibility of using the S. typhimurium plate-incorporation assay of Ames for detecting target-organ specificity with N-nitro-N-methylaniline (NMA), a compound for which the target site for tumor formation in the rat is the esophagus. The esophagus would bioactivate this compound. The compound was investigated using S9 from Aroclor-and NMA-induced rat esophagus, salivary gland and liver in the presence and absence of the co-mutagen, norharman. No response to NMA was seen with esophageal S9, even though benzopyrene produced a dose-related increase in revertants in strain TA98 and TA100. No response to NMA was seen with salivary-gland S9. A response was produced with Aroclor-induced rat-liver S9 in the presence of norharman, and with NMA-induced rat-liver S9 in the absence of norharman.