Stereological analysis of phagocytosing cells
Shcherbukhin, V.V.; Khramtsov, A.V.; Morozov, I.A.; Senchenkov, E.P.; Zemskov, V.M.
Tsitologiia 27(3): 336-341
1985
ISSN/ISBN: 0041-3771 PMID: 3887701 Document Number: 243220
Various complexes of phagocytes with adsorbed (I) and entrapped (J) particles are formed during phagocytosis. A method of stereological reconstruction is proposed that demonstrates the actual distribution of these complexes on the basis of morphometric analysis of their ultrathin sections. The principle lies on the probability simulation of section distribution for a given distribution of complexes and on the solution of the reverse problem by stepwise determination of the relative quantity of each complex type (from the most complicated to the most simple, when I = O and J = O). The stereological analysis of phagocytozing murine peritoneal macrophages revealed an absolutely different and more adequate kinetic picture of phagocytosis, as compared to the morphometric data.