Study of some properties of ABO test sera by using the method of phagocytosing erythrocytes treated with formaldehyde in vitro
Nikolov, C.
Folia Haematologica 108(6): 838-843
1981
ISSN/ISBN: 0323-4347 PMID: 6176509 Document Number: 170147
By using the method of phagocytosis of erythrocytes treated with formaldehyde against human test sera, an indirect conclusion on the quantity of IgG antibodies in the used sera was made. Combined tests of 0, A1 and B blood groups with erythrocytes treated with formaldehyde against test sera 0 (.alpha. + .beta.), A (.beta.) and B (.alpha.) and AB (0) sera from nonimmunized and immunized with anatoxin from Staphylococcus aureus donors were made. The strongest phagocytosis was observed when A1 and B erythrocytes with 0 (.alpha. + .beta.) sera and A1 erythrocytes with AB (0) sera from immunized with anatoxin donors were combined. A weaker phagocytosis took place when erythrocytes noncompatible with the ABO-system A1 and B were combined with the B (.alpha.) A (.beta.) test sera, and the weakest, when erythrocytes compatible with the ABO system and sera were combined. The same correlation was established by using ABO test sera diluted with an equal volume of saline and heated at 70.degree. C for 10 min.