Effect of NAD and ADP on glycolysis in the kidneys and liver of rats during ischemia
Tamarina, N.Z.; Chumakov, V.N.; Lystsova, G.V.
Ukrainskii Biokhimicheskii Zhurnal 56(1): 46-52
1984
ISSN/ISBN: 0201-8470 PMID: 6710614 Document Number: 239228
Experiments on albino rats have shown that kidney ischemia and its simulation by the anaerobic incubation of postmitochondrial kidney homogenate fraction without a substrate induce a considerable damage of the glycolytic system at the stage of the glucoso-6-phosphate transformation into fructoso-1.6-diphosphate and a less pronounced damage in the fructoso-1.6-diphosphate transformation into lactate. Administration of adenosine diphosphate (ADP) and nicotinamide adenine dinucleotide (NAD) to rats before kidney vessel occlusion or their addition to the postmitochondrial fraction before the anaerobic incubation without a substrate decreased a degree of the glycolytic system damage. The damage of the glycolytic system and protective action of NAD are also detected under simulation of liver ischemia. Possible mechanisms of the ischemic damage in the glycolytic liver and kidney tissue system are discussed.