Top Abstract Purchase Related
Home
>
Node
>
Subnode

Microsomal glucose 6-phosphate dehydrogenase in the rat testis--an testis--specific isoenzyme?

Wagner, H.J.; Schülke, B.

Biomedica Biochimica Acta 43(1): 3-9

1984

ISSN/ISBN: 0232-766X
PMID: 6721876
Document Number: 237964
Mammalian tissues contain cytosolic and microsomal glucose 6-phosphate dehydrogenase (G6PD). In the present study it was examined whether the microsomal G6PD in liver and testis of the rat are homologous. Kinetic parameters and immunological reactivity were estimated using rabbit antiserum against mice erythrocyte G6PD. Kinetic parameters allow to distinguish cytosolic and microsomal G6PD in rat liver but not in testis, whereas the immunological properties of the testis enzymes are different. The cytosolic G6PDs from liver and testis of the rat are identical enzymes having extremely similar properties to mice erythrocyte G6PD. The microsomal G6PD of both organs are not homologous enzymes. Testicular microsomal G6PD is likely to be an organ specific G6PD-isoenzyme.

Document emailed within 1 workday
Secure & encrypted payments

Van der Hoorn, F.A. 1992: Identification of the testis c-mos promoter: specific activity in a seminiferous tubule-derived extract and binding of a testis-specific nuclear factor Oncogene 7(6): 1093-1099
Bel'chenko, D.I.; Khanina, N.I.; Kapustin, A.V. 1983: Glucose-6-phosphate dehydrogenase and lactate dehydrogenase activities and lactate dehydrogenase isoenzyme spectrum in human myocardium after sudden death caused by acute alcohol intoxication and ischemic heart disease Voprosy Meditsinskoi Khimii 29(3): 80-82
Tokarev, Y.N.; Chernyak, N.B.; Batishchev, A.I.; Lamzina, N.V.; Alexeyev, G.A. 1979: Study of the electrophoretic and kinetic properties of erythrocyte glucose-6-phosphate dehydrogenase in hereditary enzyme deficiencies. Description of a new variant of glucose-6-phosphate dehydrogenase: the Gd Kremenchug Nouvelle Revue Francaise d'Hematologie 20(4): 557-564
Li, F.; Zou, Y.-g.; Guo, W.-b.; Li, J.-p.; He, J.-c.; Li, J.-m.; Mao, X.-m. 2014: The aged testis. a good model to find proteins involved in age-related changes of testis by proteomic analysis Journal of Reproductive Medicine 59(1-2): 31-38
Johansen, T.E.; Kummen, O. 1987: Malignancy, fertility and undescended testis. Report of a patient with cancer of a solitary testis, and a review of the literature Tidsskrift for den Norske Laegeforening: Tidsskrift for Praktisk Medicin Ny Raekke 107(3): 234-5 239
Khlebarova, M.; Dinkov, L.; Tsoncheva, A. 1971: Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activity in animals with experimental ketosis Eksperimentalna Meditsina i Morfologiia 10(3): 157-163
Drel', K.A.; Shelest, I.P. 1976: Lactate dehydrogenase and glucose-6-phosphate dehydrogenase activity in the erythrocytes in diabetes mellitus Voprosy Meditsinskoi Khimii 22(4): 469-472
Stabrovskaia, V.I.; Braun, A.D. 1978: Changes in lactate dehydrogenase and glucose-6-phosphate dehydrogenase activity in the course of muscle alteration Tsitologiia 20(9): 1040-1045
Tsapok, P.I.; Meshchishen, I.F. 1978: Adenosine triphosphatase, lactate dehydrogenase and glucose-6-phosphate dehydrogenase activity of pancreas of thyroidectomized rats Ukrainskii Biokhimicheskii Zhurnal 50(3): 322-324
Kudriavtseva, G.V.; Denisova, I.I. 1980: Determination of half-life time for the key enzymes of the pentose phosphate pathway--glucose-6-phosphate dehydrogenase and transketolase Biokhimiia 45(1): 118-123
Uygur, M.C.; Arik, A.I.; Erol, D.; Ozer, E.; Ustün, H. 1999: Quantitative evaluation of biopty gun testis needle biopsy. Correlation between biopsy score of varicocele-bearing testis and sperm count Journal of Reproductive Medicine 44(5): 445-449
Goldenberg, D.M.; Bhan, R.D.; Pavia, R.A. 1971: In vivo human-hamster somatic cell fusion indicated by glucose 6-phosphate dehydrogenase and lactate dehydrogenase profiles Cancer Research 31(8): 1148-1152
Fomina, M.P. 1973: Activity of hexokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in intact and sympathectomized rat testes exposed to hormones Voprosy Meditsinskoi Khimii 19(6): 582-586
Nishio, H.; Matsui, H.; Etoh, S.; Moia, L.J.; Tokuda, M.; Itano, T.; Hatase, O. 1992: Identification of testis specific calcineurin beta subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence Biochemical and Biophysical Research Communications 182(1): 34-38
Sikorska, M.; Gorzkowski, B.; Szumańska, G.; Smialek, M. 1975: Incorporation of 14C glucose into glycogen and glucose-6--phosphate dehydrogenase activity in rat brain following carbon monoxide intoxication Neuropatologia Polska 13(3-4): 397-404
Neeley, W.E. 1972: Simple automated determination of serum or plasma glucose by a hexokinase-glucose-6 -phosphate dehydrogenase method Clinical Chemistry 18(6): 509-515
Maĭdanova, N.V. 1973: Glucose-6-phosphate dehydrogenase, transketolase and glucose-6-phosphatase activity in the rat liver with a varying provision of corticosteroids and ACTH Voprosy Meditsinskoi Khimii 19(4): 389-394
Sanyal, S.; Patra, P.B.; Biswas, N.M. 1978: Histochemical studies on the long term prostaglandin treatment on adrenal delta5-3beta-hydroxysteroid dehydrogenase and glucose-6-phosphate dehydrogenase activities in rats pretreated with estradiol dipropionate Anatomischer Anzeiger 144(3): 214-219
Meijer, D.; Hermans, A.; von Lindern, M.; van Agthoven, T.; de Klein, A.; Mackenbach, P.; Grootegoed, A.; Talarico, D.; Della Valle, G.; Grosveld, G. 1987: Molecular characterization of the testis specific c-abl mRNA in mouse EMBO Journal 6(13): 4041-4048
Bedino, S.; Testore, G. 1998: Rat testis mitochondrial aldehyde dehydrogenase: kinetic evidence for a direct reaction between capronaldehyde and enzyme-bound NAD+ in the presence of Mg2+ ions Italian Journal of Biochemistry 47(2): 91-100