Top Abstract Purchase Related
Home
>
Node
>
Subnode

Method of isolating and controlling fluorescent Fab fragments of antibodies against horse serum proteins

Barban, I.S.; Pantiukhina, A.N.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2: 102-105

1984

ISSN/ISBN: 0372-9311
PMID: 6424360
Document Number: 237032
For the first time the lyophilized fluorescent Fab-fragments of rabbit antibodies to horse serum protein, suitable for detecting different antigens and antibodies to these antigens, have been obtained by the specially developed method. The criteria to be used in the control of the antispecific fluorescent fragments of antibodies have been described and the methods of their control before and after lyophilization have been developed. The use of the antispecific fluorescent Fab-fragments of antibodies has been shown to considerably accelerate and simplify the indirect immunofluorescent assay.

Document emailed within 1 workday
Secure & encrypted payments

Nagasawa, T. 1982: Application of immunological tests of the serum and urine--detection of the serum antigens and antibodies and fluorescent antibody technic for the renal tissue Rinsho Byori. Japanese Journal of Clinical Pathology Suppl 49: 81-87
Antonenko, V.T.; Korolev, I.N. 1985: Use of the method of ultrafiltration through UAM Vladipor semipermeable membranes for isolating low-molecular blood serum substances Laboratornoe Delo 10: 590-591
Belozerov, A.P. 1989: A method of isolating C1q from human serum and its use in the solid-phase immunoenzyme determination of immune complexes Laboratornoe Delo 3: 24-26
Gutman, N.R.; Kul'berg, A.I. 1973: Increase in the specific activity of anti-influenza serum by the use of antiglobulin antibodies and their bivalent F(ab')2-fragments Biulleten' Eksperimental'noi Biologii i Meditsiny 76(7): 71-74
Iwamatsu, A. 1991: An effective method to prepare peptides fragments from proteins on PVDF membrane filter for micro amino acid sequencing Seikagaku. Journal of Japanese Biochemical Society 63(2): 139-143
Gruzunov, I.A.; Lukicheva, T.I. 1994: Correctness and reproducibility of the fluorescent method in the determination of mass concentration of human serum albumin Klinicheskaia Laboratornaia Diagnostika 5: 25-27
Jefferis, R.; Butwell, A.J.; Clamp, J.R. 1975: Studies on human IgD myeloma proteins. Carbohydrate composition of intact proteins and some proteolytic fragments Clinical and Experimental Immunology 22(2): 311-315
Huang, Q.; Wu, T.; Liang, J.; Yang, K.; Jin, D. 2009: Comparison of whole bone marrow culture method and density gradient centrifugation method of isolating hBMSCs Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 23(11): 1360-1364
Barrows, P.L. 2015: Alternative route for controlling wild horse and burro population Journal of the American Veterinary Medical Association 247(12): 1359
Lee, C.H.; Olson, L.D.; Rodabaugh, D.E. 1976: Influence of medication on development of serum antibody to swine dysentery as detected with indirect fluorescent antibody method American Journal of Veterinary Research 37(10): 1159-1163
Lubran, M.M. 1978: The measurement of total serum proteins by the Biuret method Annals of Clinical and Laboratory Science 8(2): 106-110
Klintsova, S.P.; Kiriukhin, I.F.; Troitskiĭ, G.V. 1982: Method of analyzing serum proteins in nonionic detergents Ukrainskii Biokhimicheskii Zhurnal 54(6): 669-673
Fialová, L.; Bartos, A.; Soukupová, J.; Svarcová, J.; Ridzon, P.; Malbohan, I. 2009: Synergy of serum and cerebrospinal fluid antibodies against axonal cytoskeletal proteins in patients with different neurological diseases Folia Biologica 55(1): 23-26
Ivanova, V.T.; Zakstel'skaia, L.I.; Trushinskaia, G.N.; Shenderovich, S.F.; Liubovtseva, O.V. 1983: Use of agarose for isolating influenza virus proteins retaining a high immunogenicity Voprosy Virusologii 28(4): 32-35
Alter, H.J.; Polesky, H.F.; Holland, P.V. 1972: False positive tests for hepatitis-associated antigen in blood donors caused by antibodies to ruminant serum proteins Journal of Immunology 108(2): 358-369
Filatova, T.N.; Gamzulina, L.N.; Zamiatina, T.A. 1995: The use of silica gel-based ion-exchange sorbents for isolating one of the iron-dependent proteins of meningococci Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 6: 19-20
Trushinskaia, G.N.; Simonov, V.I.; Makarova, N.E.; Nosik, D.N.; Kushch, A.A.; Gibadulin, R.A. 1992: The immunoenzyme test system for detection of HIV-1 antigens based on using immune polyclonal anti-HIV serum and monoclonal antibodies against gene GAG HIV-1 proteins Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 1992(9-10): 43-47
Trubitsyna, N.V.; Mikheev, A.M.; Berezin, V.A.; Efimov, O.E.; Privalova, L.I. 1989: An analysis of blood serum proteins in chronic alcoholism patients by a cross immunoelectrophoresis method Voprosy Meditsinskoi Khimii 35(3): 133-136
Lupinovich, V.L.; Kopylova, N.V. 1980: Blood serum proteins studied by a method of polyacrylamide gel disc electrophoresis in children with phenylketonuria Voprosy Pitaniia 6: 24-26
Wranicz, A. 1971: Clinical usefulness of detection of serum antinuclear antibodies by the immunofluorescence method Przeglad Dermatologiczny 58(6): 685-690