The measurement of total serum proteins by the Biuret method
Lubran, M.M.
Annals of Clinical and Laboratory Science 8(2): 106-110
1978
ISSN/ISBN: 0091-7370 PMID: 345944 Document Number: 136886
The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer's law is obeyed to at least 10 g per dl. Several stable biuret reagents are available. Hemoglobin is the only important cause of interference which cannot be minimized by use of a sample blank. The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of the serum proteins and to the use of a certified albumin standard.