Liquid chromatographic determination of prednisolone in tablets and bulk drugs: interlaboratory study
Brower, J.F.
Journal - Association of Official Analytical Chemists 67(4): 674-676
1984
ISSN/ISBN: 0004-5756 PMID: 6469892 Document Number: 231350
A normal phase liquid chromatographic (LC) method for the determination of prednisolone in tablets and bulk drugs was studied by 7 analysts. An LC system, consisting of a methanol-water-ethylene dichloride-acetic acid mobile phase and a silica column, was used to analyze bulk drugs, individual tablets and composite samples. Analysts were supplied with 16 samples, including simulated formulations, composites of commercial tablets, intact tablets and bulk drug substances. Results agreed with those obtained by the author. The coefficients of variation of the analysts' results ranged from 1.34% for bulk drugs to 2.14% for tablet composites. The LC method is suggested as an alternative to the official AOAC [Association of Official Analytical Chemists] and USP XX blue tetrazolium colorimetric methods.