The in vivo effect of benzamide and phenobarbital on liver enzymes: poly (ADP-ribose) polymerase, cytochrome P-450, styrene oxide hydrolase, cholesterol oxide hydrolase, glutathione S-transferase and UDP-glucuronyl transferase
Griffin, M.J.; Kirsten, E.; Carubelli, R.; Palakodety, R.B.; Mclick, J.; Kun, E.
Biochemical and Biophysical Research Communications 122(2): 770-775
1984
ISSN/ISBN: 0006-291X PMID: 6087814 Document Number: 230722
Rats fed a synthetic diet containing 0.25% benzamide, 0.1% phenobarbital, separately or in combination, for 2 wk showed a significant augmentation in the activity of nuclear poly(ADP-ribose) polymerase as well as changes in various nuclear, microsomal and cytosolic liver enzymes involved in the metabolism of xenobiotics. A selective depression of microsomal styrene oxide hydrolase activity by benzamide feeding, and a contrasting augmentation by phenobarbital, were confirmed by immunological titration of the enzyme-protein content suggesting actual enzyme repression and induction. The NAD content of these livers is not altered significantly as a result of benzamide and phenobarbital feeding, indicating that the changes in enzymes are not a result of non-specific toxic effects.