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Quantitative determination of total mitochondrial protein with Coomassie

Aliverdieva, D.A.; Shol'ts, K.F.

Prikladnaia Biokhimiia i Mikrobiologiia 20(6): 823-830

1984

ISSN/ISBN: 0555-1099
PMID: 6522359
Document Number: 230609
The mechanism of Coomassie interaction with proteins was being studied. Under certain conditions the range of detectible polypeptides can be wider. For protein analysis in mitochondria we have suggested a new component ratio for the Bradford reagent. A correction for mitochondrial protein was determined, which should be made on using bovine serum albumin as a standard.

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