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Detection of filarial antigen in urine of humans with Wuchereria bancrofti infection by immunoradiometric assay

Reddy, M.V.; Harinath, B.C.; Hamilton, R.G.

Indian Journal of Experimental Biology 22(10): 515-519

1984

ISSN/ISBN: 0019-5189
PMID: 6397434
Document Number: 228783
Human urine samples of different groups were analyzed for the presence of filarial antigen by immuno-radiometric assay (IRMA) using 125I-Rabbit IgG antibodies to Brugia malayi antigen. Six of 10 microfilaremia and 1 of 5 clinical filariasis had detectable antigen, while none of the endemic and non-endemic normals (n = 12) were positive. The effect of heat-acid treatment on the detectability of antigen in the urine and serum was explored. Heat-acid treatment in general did not reduce the level of filarial antigen in the urine sample while there was a measurable reduction in the antigen levels in the sera.

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