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A method for routine estimation of vitamin D activity in human and bovine milk

Parviainen, M.T.; Koskinen, T.; Ala-Houhala, M.; Visakorpi, J.K.

Acta Vitaminologica et Enzymologica 6(3): 211-219

1984

ISSN/ISBN: 0300-8924
PMID: 6524580
Document Number: 224022
Vitamin D metabolites were estimated in whole cow's milk and human milk by a method involving lipid extraction, precipitation with cold methanol and ether, alkaline backwash to reduce interfering lipids, reverse-phase purification, additional silica purification for vitamin D, analytical high-performance liquid chromatography and separate sensitized protein-binding tests for vitamin D and 25-hydroxyvitamin D. There was good recovery of added vitamin D in whole milk. Antirachitic activity of metabolites was about 10 to 50 IU/litre.

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