Organotypic culture of normal and neoplastic laryngeal mucosa
Bodo, M.; Pitzurra, M.; Sensini, A.; Maurizi, M.; Paludetti, G.; Fronticelli, F.
Bollettino della Societa Italiana di Biologia Sperimentale 60(3): 611-616
1984
ISSN/ISBN: 0037-8771 PMID: 6712829 Document Number: 222643
Mucous membrane's phragments from human normal and neoplastic larynx have been maintained in a organ culture system. A set of cultures has been prepared for optical microscope studies and a parallel set for incorporation studies using 3H Thymidine and 3H Leucine. The results show that there is a good incorporation of 3H Thymidine and a greater incorporation of 3H Leucine at the 7th day due to a differentiation in vitro with a new synthesis of cheratin. These data agree with the morphologic evidence of considerable horny structures in the pathological mucous membrane after 11 days at culture.