Regulating role of various amino acids in development of apoptosis in organotypic culture of the nervous and lymphoid tissue
Chalisova, N.I.; Pennijainen, V.A.; Haase, G.
Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova 88(5): 627-633
2002
ISSN/ISBN: 0869-8139 PMID: 12136731 Document Number: 544966
The effect of aminoacids L-arginin, L-lysine, L-asparagin was investigated in organotypic tissue culture of brain cortex and spleen of 1-day old rats. The aminoacids in concentrations 0.05 and 0.1 ng/ml are active, inducing a less intensive growth zone as compared to the control, excluding the effect of asparagin on lymphoid tissue. Method of fluorescent staining shows a negative correlation between growth zone size and apoptotic cell number. The nerve and lymphoid tissue express apoptosis in response to aminoacids.